Xyloglucan fucosyltransferase, an enzyme involved in plant cell wall biosynthesis

Perrin R.M.; Derocher A.E.; Bar-Peled M.; Zeng W.; Raikhel N.V.; Keegstra, K; Norambuena, L; Orellana, A

Keywords: sequence, acid, enzyme, membrane, resistance, animals, expression, cells, protein, plant, cell, disease, electrophoresis, polysaccharides, conformation, transduction, pisum, gel, hemicellulose, cellulose, fucosyltransferase, wall, pectin, signal, drug, molecular, data, article, arabidopsis, carbohydrate, mammalia, analysis, activity, glucan, xylene, xyloglucan, tags, polyacrylamide, amino, priority, peas, nonhuman, journal, DNA,, Complementary, Genes,, sativum, unclassified, Cloning,, COS, Xylans, Expressed, Fucosyltransferases, Glucans

Abstract

Cell walls are crucial for development, signal transduction, and disease resistance in plants. Cell walls are made of cellulose, hemicelluloses, and pectins. Xyloglucan (XG), the principal load-bearing hemicellulose of dicotyledonous plants, has a terminal fucosyl residue. A 60-kilodalton fucosyltransferase (FTase) that adds this residue was purified from pea epicotyls. Peptide sequence information from the pea FTase allowed the cloning of a homologous gene, AtFT1, from Arabidopsis. Antibodies raised against recombinant AtFTase immunoprecipitate FTase enzyme activity from solubilized Arabidopsis membrane proteins, and AtFT1 expressed in mammalian COS cells results in the presence of XG FTase activity in these cells.

Más información

Título de la Revista: SCIENCE
Volumen: 284
Número: 5422
Editorial: AMER ASSOC ADVANCEMENT SCIENCE
Fecha de publicación: 1999
Página de inicio: 1976
Página final: 1979
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-0033580981&partnerID=q2rCbXpz
DOI:

10.1126/science.284.5422.1976

Notas: ISI