Influence of embryonic conditioned media upon the endometrial ?-adrenergic receptor

Bruzzone M.E.; Benitez D.A.; Castellón E.A.; Fabres M.C.; Zegers-Hochschild, F

Keywords: development, ovulation, expression, transcription, culture, protein, cell, pregnancy, progesterone, chain, beta, embryo, estradiol, humans, infertility, implantation, human, male, receptor, polymerase, time, biopsy, adult, female, adrenergic, endometrium, cycle, article, techniques, incubation, controlled, reverse, study, 1, 3, Reaction, Receptors,, 2, normal, Cells,, Cultured, Transfer, medium, Media,, Conditioned, Adrenergic,, menstrual, nidation

Abstract

The main objective of this study was to evaluate the influence of the products secreted by the human embryo upon the three subtypes of ?-AR (?1, ?2, ?3). Cell cultures were developed using endometrial biopsies, taken on day 7 after ovulation, from four healthy women <35 years of age, with regular cycles and infertility due only to male factors. Embryos from women with a normal uterine cavity and endometrial lining were incubated until they reached the 4-cell stage, before being transferred to their mother's uterus. Culture media for embryo incubation were derived from two groups: (i) embryos that achieved pregnancy, (ii) embryos which failed to implant. Control and experimental endometrial cell culture plates were treated with the two embryo culture media, with or without 10-9 mol/l oestradiol and 10-7 mol/l progesterone for 48 h. Expression of the three subtypes of ?-AR was assessed by RT-PCR. ?1-AR was expressed in both control and experimental plates; ?2-AR was expressed only in plates incubated with embryonic culture media of embryos which achieved pregnancy, in both hormonal conditions, with or without oestradiol and progesterone. ?3-AR was not expressed in any condition. Thus secretory products of human embryos may influence gene expression of ?2-AR concentrations in the human endometrium, and this subtype of ?-AR may be involved in implantation.

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Título de la Revista: REPRODUCTIVE BIOMEDICINE ONLINE
Volumen: 11
Número: 1
Editorial: ELSEVIER SCI LTD
Fecha de publicación: 2005
Página de inicio: 58
Página final: 63
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-21844459750&partnerID=q2rCbXpz