Resistance to anti-retroviral therapy in Chilean patients with HIV-1 from 2002 to 2005 Resistencia a la terapia antirretroviral en pacientes infectados con el virus VIH-1 en Chile 2002-2005

Afani S A.; Orellana R L.; Duarte J P.; Acevedo M W.; Morales B O.; Wolff R M.; Vasquez, P; beltran, C

Keywords: enzymology, chile, dna, resistance, therapy, infections, hiv, infection, immunodeficiency, mutation, virus, humans, human, male, genetics, agents, polymerase, inhibitor, aged, protease, agent, adult, female, genotype, rna, proteinase, drug, inhibitors, active, article, load, viral, lymphocyte, hiv-1, cd4, reverse, studies, study, 1, multidrug, count, middle, RNA,, effect, virology, Resistance,, Retrospective, directed, Therapy,, Transcriptase, highly, anti, Anti-HIV, Antiretroviral, Multiple,

Abstract

Background: Resistance limits the effectiveness of anti-retroviral therapy. In Chile, there is free access to highly active anti-retroviral therapy since 2001, but there is no information about the frequency of mutations associated to drug resistance. Aim: To determine the most common mutations associated to anti-retroviral drug resistance in Chile. Materials and Methods: Retrospective study of 710 genotype analysis coming from 568 patients aged 22 to 70 years (85% males) with virological failure. The analysis was performed using a commercially available sequencing kit (Trugene HIV-1 genotypic assay from Bayer S.A). Results: Mean CD4+ cell count and viral load were 154 cells/ μl and 228784 RNA copies/ml, respectively. The frequency of resistance to nucleoside RT inhibitors (NRTI), non nucleoside RT inhibitors (NNRTI) and protease inhibitors (PI) was 71%, 62% and 22%, respectively. The most common mutations found were T215Y (46%), L10F (44%), M184V (38%), K103N (35%) and M41L (32%). Fifty five percent of mutations corresponded to the TAM (thymidine analogue mutations) group. Multiresistance was 47% to NNRTI, 7% to NRTI, 4% to PI and 0.7% to all groups. During the four years of the study, there was a significant increase in NNRTI resistance. Conclusions: These data provides important information about the epidemiology of drug resistance mutations and should help to design new HAART strategies.

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Título según SCOPUS: Resistance to anti-retroviral therapy in Chilean patients with HIV-1 from 2002 to 2005 Resistencia a la terapia antirretroviral en pacientes infectados con el virus VIH-1 en Chile 2002-2005
Título de la Revista: REVISTA MEDICA DE CHILE
Volumen: 135
Número: 10
Editorial: SOC MEDICA SANTIAGO
Fecha de publicación: 2007
Página de inicio: 1237
Página final: 1244
Idioma: eng
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-37049035353&partnerID=q2rCbXpz
Notas: SCOPUS