Article
ELECTRONIC JOURNAL OF BIOTECHNOLOGY  (2009)

Measuring ?-Galactosidase activity at pH 6 with a differential pH sensor

Acevedo, C; Young M.E.; Stach M.H.; Amtmann, A; Huebner, H; Buchholz R.; Reyes, J.G.

Keywords: enzyme, culture, aging, extract, cell, markers, mechanism, beta, sensors, ph, measurement, article, marker, cytochemistry, delta, activity, cellular, effects, activities, differential, galactosidase, enzymatic, Cytochemical, milli

Abstract

The ?-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure ?-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min. © 2009 by Pontificia Universidad Católica de Valparaíso.

Más información

Título de la Revista: ELECTRONIC JOURNAL OF BIOTECHNOLOGY
Volumen: 12
Número: 2
Editorial: UNIV CATOLICA DE VALPARAISO
Fecha de publicación: 2009
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-66849142198&partnerID=q2rCbXpz