Portal del Investigador
Effects of aristotelia chilensis berry juice on cyclooxygenase 2 expression, NF-?B, NFAT, ERK1/2 and PI3K/Akt activation in colon cancer cells Efecto de un concentrado del fruto de aristotelia chilensis sobre la expresión de cicloxigenasa 2, NF-k{cyrillic}B, NFAT, ERK1/2 y activación de PI3K/Akt en células de cancer de colon
Keywords: growth, enzyme, activation, expression, phosphorylation, transcription, binding, culture, protein, cell, gene, colon, alpha, cancer, viability, transduction, strain, juice, fruit, antiinflammatory, signal, rna, cyclooxygenase, drug, article, kinase, factor, enhancer, anthocyanin, antineoplastic, omega, kappa, immunoglobulin, activity, concentration, chilensis, berry, controlled, delphinidin, cyanidin, aristotelia, c, study, 1, 3, response, 2, Messenger, I, o, unclassified, 5, activated, b, transforming, phosphatidylinositol, mitogen, caco, luciferase, 3,5, glucoside, fos, NFAT, sambubioside
Abstract
Aristotelia chilensis is a native berry from southern Chile with a high content of anthocyanins, compounds that exhibit antioxidant and anti-inflammatory properties. In the present study, we evaluated the effects of A. chilensis berry juice on cyclooxygenase (COX)-2 expression, intracellular signaling pathways, and cell viability in colon cancer cells. The treatment of Caco-2 cells with A. chilensis diluted juice for 24 h reduced the protein and mRNA expression of COX-2, as well as the TNF-?-induced NF-k{cyrillic}B luciferase activity and NFAT activation. In contrast, 4 h after administration, A. chilensis transiently reduced the cytoplasmic Ik{cyrillic}B? levels and increased ERK1/2 and Akt phosphorylation as well as c-fos expression. At concentrations that reduced COX-2 expression, A. chilensis did not affected Caco-2 cell viability. Our results suggest a potential anti-carcinogenic and anti-inflammatory effect of A. chilensis. © 2011 Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas.
Más información
| Título de la Revista: | BOLETIN LATINOAMERICANO Y DEL CARIBE DE PLANTAS MEDICINALES Y AROMATICAS |
| Volumen: | 10 |
| Número: | 6 |
| Editorial: | MS-EDITIONS |
| Fecha de publicación: | 2011 |
| Página de inicio: | 543 |
| Página final: | 552 |
| URL: | http://www.scopus.com/inward/record.url?eid=2-s2.0-83055173915&partnerID=q2rCbXpz |