Estudio de patrones de metilación génica en tumores del tubo digestivo

Roa S, Juan Carlos; GARCÍA M, PATRICIA; Melo A, Angélica; Tapia E, Oscar; Villaseca H, Miguel; ARAYA O, JUAN CARLOS; GUZMÁN G, PABLO

Abstract

"Background: The loss of tumor suppresor gene function damages the defensive mechanisms that protect the indemnity of genetic material. Promoter gene methylation is one of the inactivation mechanisms of suppressor genes. Aim: To study the methylation pattern of a group of genes in biopsy samples of gastrointestinal tumors. Material and methods: Forty eight gastric, 25 gallbladder, 24 colon and 6 pancreas cancer biopsy samples were randomly selected. The methylation pattern of CDH1, FHIT, CDKN2A, APC and MLH1 genes, was studied using a specific polymerase chain reaction test for methylation. Demographic, morphological and follow up variables of patients bearing the tumors were also analyzed. Results: The general methylation frequency of CDH1, FHIT, CDKN2A, APC and MLH1 genes was 64.1, 56, 39.8, 18.1 and 34% respectively. In gastric cancer samples there was a correlation between APC gene methylation and well differentiated tumors; between CDH1 methylation and Lauren diffuse type and the presence of three or more metastasic lymph nodes; between FHIT, CDKN2A and CDH1 gene methylation and male gender. In

Más información

Título según SCIELO: Estudio de patrones de metilación génica en tumores del tubo digestivo
Título de la Revista: REVISTA MEDICA DE CHILE
Volumen: 136
Número: 4
Editorial: SOC MEDICA SANTIAGO
Fecha de publicación: 2008
Página de inicio: 451
Página final: 458
Idioma: es
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872008000400005&lng=en&nrm=iso&tlng=en
DOI:

10.4067/S0034-98872008000400005

Notas: SCIELO