A coumarinylaldoxime as a specific sensor for Cu2+ and its biological application

Garcia-Beltran, O; Cassels, B.K.; Mena N.; Núñez M.T.; Yanez, O.; Caballero, J

Abstract

In this Letter we present a new probe, (E)-7-(diethylamino)-2-oxo-2H-chromene-3-carbaldehyde oxime (JB), which can detect Cu2+ ions in HEPES buffer under physiological conditions. Benesi-Hildebrand and Job plots demonstrate that the stoichiometry of the Cu2+ complex formed is 2:1. Possible interference with other analytes was examined, and the decrease of the fluorescence of JB at 510 nm when it reacts with Cu2+ was shown to be highly selective. This probe accumulates in the plasmalemma of human neuroblastoma SH-SY5Y cells. Molecular dynamics (MD) simulations revealed thatJB interacts with the lipid bilayer at the level of the glycerol moieties. (C) 2013 Elsevier Ltd. All rights reserved.

Más información

Título según WOS: A coumarinylaldoxime as a specific sensor for Cu2+ and its biological application
Título según SCOPUS: A coumarinylaldoxime as a specific sensor for Cu2+ and its biological application
Título de la Revista: TETRAHEDRON LETTERS
Volumen: 55
Número: 4
Editorial: PERGAMON-ELSEVIER SCIENCE LTD
Fecha de publicación: 2014
Página de inicio: 873
Página final: 876
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0040403913021084
DOI:

10.1016/j.tetlet.2013.12.033

Notas: ISI, SCOPUS