The Structure-Function Linkage Database

Akiva E.; Brown, S; Almonacid D.E.; Barber A.E.; Custer A.F.; Hicks M.A.; Huang, C.C.; Lauck F.; Mashiyama S.T.; Meng E.C.; Mischel D.; Morris J.H.; Ojha, S; Schnoes A.M.; Stryke D.; et. al.

Abstract

The Structure-Function Linkage Database (SFLD, http://sfld.rbvi.ucsf.edu/) is a manually curated classification resource describing structure-function relationships for functionally diverse enzyme superfamilies. Members of such superfamilies are diverse in their overall reactions yet share a common ancestor and some conserved active site features associated with conserved functional attributes such as a partial reaction. Thus, despite their different functions, members of these superfamilies 'look alike', making them easy to misannotate. To address this complexity and enable rational transfer of functional features to unknowns only for those members for which we have sufficient functional information, we subdivide superfamily members into subgroups using sequence information, and lastly into families, sets of enzymes known to catalyze the same reaction using the same mechanistic strategy. Browsing and searching options in the SFLD provide access to all of these levels. The SFLD offers manually curated as well as automatically classified superfamily sets, both accompanied by search and download options for all hierarchical levels. Additional information includes multiple sequence alignments, tab-separated files of functional and other attributes, and sequence similarity networks. The latter provide a new and intuitively powerful way to visualize functional trends mapped to the context of sequence similarity.

Más información

Título según WOS: The Structure-Function Linkage Database
Título de la Revista: NUCLEIC ACIDS RESEARCH
Volumen: 42
Número: D1
Editorial: OXFORD UNIV PRESS
Fecha de publicación: 2014
Página de inicio: D521
Página final: D530
Idioma: English
URL: http://nar.oxfordjournals.org/lookup/doi/10.1093/nar/gkt1130
DOI:

10.1093/nar/gkt1130

Notas: ISI