Fat intake leads to differential response of rat adipocytes to glucose, insulin and ascorbic acid

Garcia-Diaz, DF; Campion, J.; Arellano AV; Milagro, FI; Moreno-Aliaga, MJ; Martinez, JA

Abstract

Antioxidant-based treatments have emerged as novel and interesting approaches to counteract fat accumulation in obesity and associated metabolic disturbances. Adipocytes from rats that were fed on chow or high-fat diet (HFD) for 50 d were isolated (primary adipocytes) and incubated (72 h) on low (LG; 5.6 mmol/L) or high (HG; 25 mmol/L) glucose levels, in the presence or absence of 1.6 nmol/L insulin and 200 mu mol/L vitamin C (VC). Adipocytes from HFD-fed animals presented lower insulin-induced glucose uptake, lower lactate and glycerol release, and lower insulin-induced secretion of some adipokines as compared with controls. HG treatment restored the blunted response to insulin regarding apelin secretion in adipocytes from HFD-fed rats. VC treatment inhibited the levels of nearly all variables, irrespective of the adipocytes' dietary origin. The HG treatment reduced adipocyte viability, and VC protected from this toxic effect, although more drastically in control adipocytes. Summing up, in vivo chow or HFD intake determines a differential response to insulin and glucose treatments that appears to be dependent on the insulin-resistance status of the adipocytes, while VC modifies some responses from adipocytes independently of the previous dietary intake of the animals.

Más información

Título según WOS: Fat intake leads to differential response of rat adipocytes to glucose, insulin and ascorbic acid
Título de la Revista: EXPERIMENTAL BIOLOGY AND MEDICINE
Volumen: 237
Número: 4
Editorial: SAGE PUBLICATIONS LTD
Fecha de publicación: 2012
Página de inicio: 407
Página final: 416
Idioma: English
DOI:

10.1258/ebm.2011.011317

Notas: ISI - ISI