Abstract

A genetic polymorphism detected by the O'Farrell two-dimensional technique (isoelectric focusing and SDS-PAGE) of the murine sex-limited protein (Slp) is described and shown to map to the H-2 complex. The Slp charge variation was found to be in the gamma chains. Inbred strains carrying the H-2w7 and H-2wr7 haplotypes, which are derived from a wild mouse, had Slp-gamma chains with pI = 6.55 (Slp-1b). All other inbred strains, bearing H-2j,H-2s,H-2p,H-2d,H-2u, as well as three additional Slp-constitutive wild females captured in Chile, had Slp-gamma chain with pI = 6.71 (Slp-1a).