Thermal treatment enhances the stability and biological activity of a truncated tilapia somatotropin contained in Pichia pastoris culture supernatant

Jannel Acosta; Odalys Ruiz; Yamila Carpio; Reynold Morales; Julio C. Aguila; Jorge Valdes; Eduardo Martínez; Mario P Estrada

Abstract

The importance of somatotropin as a growth promoting agent and immune-stimulator has long been recognized and its potential application in the fish farming industry has been an active research area. In the work reported here, we sought to improve the stability of a previously obtained truncated somatotropin by applying a 60 degrees C heat shock to the culture supernatant containing this molecule, and then compared its effects with and without heat shock on larval growth and immune functions. We observed that the treatment with heat shock at 60 degrees C enhanced protein stability, growth and innate immune functions in tilapia larvae. (C) 2010 Elsevier B.V. All rights reserved.

Más información

Título según WOS: Thermal treatment enhances the stability and biological activity of a truncated tilapia somatotropin contained in Pichia pastoris culture supernatant
Título de la Revista: JOURNAL OF BIOTECHNOLOGY
Volumen: 151
Número: 2
Editorial: ELSEVIER SCIENCE BV
Fecha de publicación: 2011
Página de inicio: 175
Página final: 179
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S016816561002050X
DOI:

10.1016/j.jbiotec.2010.11.017

Notas: ISI