Abstract

DNA ploidycell cycle analysis by flow cytometry is used to obtain additional information about the diagnosisprognosis of different types of cancer. However, there are several disagreements among authors about the tissue source (fresh-frozen or paraffin embedded), cellular dissociation methods (mechanical, enzymatic or other), use of different dyes, lasers, analysis software with different mathematical modelsinterpretation of results. A discussion about the different aspects that affect the study of DNA ploidycell cyclea consensus in publications is mandatory. A strict control of analysis processesdata interpretation is also necessary