Transport along the dendritic endoplasmic reticulum mediates the trafficking of GABA(B) receptors

Valenzuela JI; Jaureguiberry-Bravo, M; Salas, DA; Ramirez, OA; Cornejo, VH; Lu, HE; Blanpied, TA; couve, a

Abstract

In neurons, secretory organelles within the cell body are complemented by the dendritic endoplasmic reticulum (ER) and Golgi outposts (GOPs), whose role in neurotransmitter receptor trafficking is poorly understood. gamma-aminobutyric acid (GABA) type B metabotropic receptors (GABA(B)Rs) regulate the efficacy of synaptic transmission throughout the brain. Their plasma membrane availability is controlled by mechanisms involving an ER retention motif and assembly-dependent ER export. Thus, they constitute an ideal molecular model to study ER trafficking, but the extent to which the dendritic ER participates in GABA(B)R biosynthesis has not been thoroughly explored. Here, we show that GABA(B1) localizes preferentially to the ER in dendrites and moves long distances within this compartment. Not only diffusion but also microtubule and dynein-dependent mechanisms control dendritic ER transport. GABABRs insert throughout the somatodendritic plasma membrane but dendritic post-ER carriers containing GABABRs do not fuse selectively with GOPs. This study furthers our understanding of the spatial selectivity of neurotransmitter receptors for dendritic organelles.

Más información

Título según WOS: Transport along the dendritic endoplasmic reticulum mediates the trafficking of GABA(B) receptors
Título según SCOPUS: Transport along the dendritic endoplasmic reticulum mediates the trafficking of GABAB receptors
Título de la Revista: JOURNAL OF CELL SCIENCE
Volumen: 127
Número: 15
Editorial: COMPANY BIOLOGISTS LTD
Fecha de publicación: 2014
Página de inicio: 3382
Página final: 3395
Idioma: English
DOI:

10.1242/jcs.151092

Notas: ISI, SCOPUS