SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPY

Araya, E.; Kogan, MJ; Guell, AG; Escobar, CA; Sanz, F

Abstract

A hydrogen-terminated Si (111) surface was modified to form an aminoterminated monolayer for immobilization of streptavidin. Cleavage of an N-(omega-undecylenyl)-phthalimide covered surface using hidrazine yields an amino group-modified surface, which serves as a substrate for the attachment of biotin and subsequently streptavidin. We used surface analytical techniques to characterize the surface and to control the course of functionalization before the immobilization of streptavidin. To confirm the presence of the streptavidin Texas red on the surface two powerful techniques available in a standard biochemical laboratory are used, Fluorescence Microscopy and MALDI-TOF that allow us to detect and determine the immobilized streptavidin. This work provides an avenue for the development of devices in which the exquisite binding specificity of biomolecular recognition is directly coupled to a biosensor. In addition, we have demonstrated that MALDI-TOF and fluorescence microscopy are useful techniques for the characterization of silicon functionalized surfaces.

Más información

Título según WOS: SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPY
Título según SCOPUS: Sensing immobilized molecules of streptavidin on a silicon surface by MALDI-TOF mass spectrometry and fluorescence microscopy
Título según SCIELO: SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPY
Título de la Revista: JOURNAL OF THE CHILEAN CHEMICAL SOCIETY
Volumen: 59
Número: 2
Editorial: 2013
Fecha de publicación: 2014
Página de inicio: 2458
Página final: 2463
Idioma: English
DOI:

10.4067/S0717-97072014000200013

Notas: ISI, SCIELO, SCOPUS