Differentiation of African components of ancestry to stratify groups in a case-control study of a Brazilian urban population

Silbiger V.N.; Hirata M.H.; Luchessi, A. D.; Genvigir F.D.V.; Cerda A.; Rodrigues A.C.; Willrich M.A.V.; Arazi S.S.; Dorea E.L.; Bernik M.M.S.; Faludi A.A.; Bertolami M.C.; Santos, C; Carracedo A.; Salas, A.; et. al.

Abstract

Background: Balancing the subject composition of case and control groups to create homogenous ancestries between each group is essential for medical association studies. Methods: We explored the applicability of single-tube 34-plex ancestry informative markers (AIM) single nucleotide polymorphisms (SNPs) to estimate the African Component of Ancestry (ACA) to design a future case-control association study of a Brazilian urban sample. Results: One hundred eighty individuals (107 case group; 73 control group) self-described as white, brown-intermediate or black were selected. The proportions of the relative contribution of a variable number of ancestral population components were similar between case and control groups. Moreover, the case and control groups demonstrated similar distributions for ACA <0.25 and >0.50 categories. Notably a high number of outlier values (23 samples) were observed among individuals with ACA <0.25. These individuals presented a high probability of Native American and East Asian ancestral components; however, no individuals originally giving these self-described ancestries were observed in this study. Conclusions: The strategy proposed for the assessment of ancestry and adjustment of case and control groups for an association study is an important step for the proper construction of the study, particularly when subjects are taken from a complex urban population. This can be achieved using a straight forward multiplexed AIM-SNPs assay of highly discriminatory ancestry markers. © Copyright 2012, Mary Ann Liebert, Inc.

Más información

Título según SCOPUS: Differentiation of African components of ancestry to stratify groups in a case-control study of a Brazilian urban population
Título de la Revista: GENETIC TESTING AND MOLECULAR BIOMARKERS
Volumen: 16
Número: 6
Editorial: Mary Ann Liebert Inc.
Fecha de publicación: 2012
Página de inicio: 524
Página final: 530
Idioma: English
DOI:

10.1089/gtmb.2011.0267

Notas: SCOPUS