Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant

Vásquez AE; Manzo RA; Soto, DA; Barrientos MJ; Maldonado, AE; Mosqueira, M; Avila, A; Touma J.; Bruce E.; Harris, PR; Venegas A.

Keywords: oral immunization, AbISCO (TM)-100 (R) adjuvant, Helicobacter pylori antigen, hybrid PorA antigens, meningococcal B porin antigen, recombinant PorA

Abstract

The Neisseria meningitidis outer membrane protein PorA from a Chilean strain was purified as a recombinant protein. PorA mixed with AbISCO induced bactericidal antibodies against N. meningitidis in mice. When PorA was fused to the Helicobacter pylori HpaA antigen gene, the specific response against H. pylori protein increased. Splenocytes from PorA-immunized mice were stimulated with PorA, and an increase in the secretion of IL-4 was observed compared with that of IFN-γ. Moreover, in an immunoglobulin sub-typing analysis, a substantially higher IgG1 level was found compared with IgG2a levels, suggesting a Th2-type immune response. This study revealed a peculiar behavior of the purified recombinant PorA protein per se in the absence of AbISCO as an adjuvant. Therefore, the resistance of PorA to proteolytic enzymes, such as those in the gastrointestinal tract, was analyzed, because this is an important feature for an oral protein adjuvant. Finally, we found that PorA fused to the H. pylori HpaA antigen, when expressed in Lactococcus lactis and administered orally, could enhance the antibody response against the HpaA antigen approximately 3 fold. These observations strongly suggest that PorA behaves as an effective oral adjuvant. KEYWORDS: AbISCO TM-100® adjuvant; AbISCO TM-100®, adjuvant based on saponin; Helicobacter pylori antigen; LAL, limulus amebocyte lysate; hybrid PorA antigens; immunostimulant complex matrix; meningococcal B porin antigen; oral immunization; recombinant PorA

Más información

Título según WOS: Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant
Título según SCOPUS: Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. Pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant
Título de la Revista: Human Vaccines and Immunotherapeutics
Volumen: 11
Número: 3
Editorial: Taylor and Francis Inc.
Fecha de publicación: 2015
Página de inicio: 776
Página final: 788
Idioma: English
DOI:

10.1080/21645515.2015.1011011

Notas: ISI, SCOPUS - ISI