Monitoring Seasonal Bud Set, Bud Burst, and Cold Hardiness in Populus

Johansson, M; Takata, N; Ibáñez C; Eriksson, ME

Keywords: freezing tolerance, photoperiod, cold acclimation, populus, perennial, Bud set, Bud burst, Critical day length

Abstract

Using a perennial model plant allows the study of reoccurring seasonal events in a way that is not possible using a fast-growing annual such as Arabidopsis thaliana (Arabidopsis). In this study, we present a hybrid aspen (Populus tremula x P.tremuloides) as our perennial model plant. These plants can be grown in growth chambers to shorten growth periods and manipulate day length and temperature in ways that would be impossible under natural conditions. In addition, the use of growth chambers allows easy monitoring of height and diameter expansion, accelerating the collection of data from new strategies that allow evaluation of promoters or inhibitors of growth. Here, we describe how to study and quantify responses to seasonal changes (mainly using P.tremula x P.tremuloides) by measuring growth rate and key events under different photoperiodic cycles.

Más información

Título según WOS: Monitoring Seasonal Bud Set, Bud Burst, and Cold Hardiness in Populus
Título de la Revista: NEISSERIA MENINGITIDIS
Volumen: 1158
Editorial: Humana Press, Inc.
Fecha de publicación: 2014
Página de inicio: 313
Página final: 324
Idioma: English
DOI:

10.1007/978-1-4939-0700-7_21

Notas: ISI