Abstract

Dental pulp is a reliable source of neural crest derived stem cells. However, the use of DPSCs in regenerative techniques is sparse, due to the difficulty of the harvesting techniques, the limited amount of viable cells obtained and the risks of bacterial contamination of the DPSC cultures. Our team has developed a method to optimize the harvesting of viable DPSCs from extracted adult tooth based on a fragile fracture procedure carried out in clean room conditions.