Role of Salmonella Typhimurium small RNAs RyhB-1 and RyhB-2 in the oxidative stress response

Calderón IL, Morales EH, Collao B, Calderón PF, Chahuán CA, Acuña LG, Gil F, Saavedra CP

Abstract

As part of the response to specific stress conditions, bacteria express small molecules of non-coding RNA which maintain cellular homeostasis by regulating gene expression, commonly at the post-transcriptional level. Among these, in Salmonella enterica sv. Typhimurium, the paralog small non-coding RNAs RyhB-1 and RyhB-2 play an important role in iron homeostasis. In addition, in the present work, we show that RyhB-1 and RyhB-2 also participate in the response to hydrogen peroxide (H2O2). Deletion of RyhB-1 and/or RyhB-2 resulted in increased levels of intracellular reactive oxygen species, protein carbonylation and an altered NADH/NAD(+) ratio. Analyses of the transcriptional profiles of ryhB-1 and ryhB-2 by northern blot and qRT-PCR showed that they are induced in response to H2O2 in an OxyR-dependent manner. By using lacZ-fusions and electrophoretic mobility shift assays, we confirmed the requirement of OxyR for inducing expression of both ryhB-1 and ryhB-2. Taken together, our results support a model in which, in response to peroxide treatment, ryhB-1 and ryhB-2 are upregulated by OxyR through direct interaction with their promoter region. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

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Título según WOS: Role of Salmonella Typhimurium small RNAs RyhB-1 and RyhB-2 in the oxidative stress response
Volumen: 165
Número: 1
Fecha de publicación: 2014
Página de inicio: 30
Página final: 40
Idioma: English
DOI:

10.1016/j.resmic.2013.10.008

Notas: ISI - ISI; SCOPUS