Abstract

Introduction: Cardiac pathologies occur with an overproduction of pro inflammatory mediators. Cardiac fibroblasts (CF) measure inflammatory environments and produces extracellular matrix proteins for tissue reparation. CF’s role is quite relevant in fibrosis and inflammation associated pathologies. Ugni molinae is a Chilean native bush. Leaf extracts have proven in vivo analgesic and anti-inflammatory properties, allocated to pentacyclic triterpenoids and phenolic compounds. Genotypic variety within the species is responsible for different chemical profiles from the leaves extracts. Objective: To evaluate the effect of two different genotypes leaves extract, EET22-1 (ethanolic extract) and EAE23-2 (ethyl acetate extract) on inflammatory mediators. Methodology. Extracts were prepared from dried minced leaves from two genotypes with growing polarity solvents. Fibroblast culture was prepared from neonate rats, and kept in DMEM/F-12, FBS 10%. Cells were stimulated with 1μg/mL LPS and the extracts (0,1-1-10 y 100 μg/mL) for 24 hours. Cell viability was measured by MTT assay, protein levels of p65 and IL-1β by western blot and MMP-9 by zymography. Results. EAE23-2 (100 μg/mL) decreased cellular viability to 20%, and EET22-1 decreased it to 36% with 500 μg/mL, in relation to the untreated control. There were no significant decrease between the pre and post treated groups in relation to the LPS control in terms of p65 phosphorylation, IL-1β levels and MMP-9 activity. Conclusions. EAE23-2 showed to be more toxic than EET22-1, probably for its higher concentrations of triterpenoid compounds. The extracts did not reverted or prevented the inflammatory response induced by LPS. More experiences are necessary for MMP-9 measurement