PROMOTER WITH A REGION RICH IN CYTOSINE-GUANINE DINUCLEOTIDES, VECTORS, CELLULAR LINES, METHOD FOR PRODUCING RECOMBINANT PROTEIN

Aguillón, Juan Carlos; Molina, María Carmen; Zúñiga, Roberto; Gutiérrez, Matías; Collazo, Norberto; Teneb, Jaime

Abstract

The present invention relates to the field of genetic engineering, preferably the expression of recombinant proteins (RP). In particular, the invention relates to a promoter and variants thereof having an equal function and more than 90% sequence identity. The promoter comprises a fragment of 1147 base pairs (bp) of a first promoter, promoter of the β-actin gene of the Cricetulus griseus genome, enriched in cytosine-guanine dinucleotides (RegCG). The first promoter can be upstream of a second promoter, cytomegalovirus (CMV) promoter. The invention also relates to vectors, transfected cellular lines and a method for producing RP in mammal cells that have been transfected with vectors containing said promoter or variants thereof.

Más información

Fecha de publicación: 2019
Idioma: PROMOTOR; DINUCLEOTIDOS; VECTORES; LINEAS CELULARES; PROTEINA; RECOMBINANTE
URL: https://patentimages.storage.googleapis.com/a6/16/9d/a88c24e029eca1/EP3495487A1.pdf
DOI:

WO 2018/000105