A basic 18-amino acid peptide contains the polysulfate-binding domain responsible for activation of the boar proacrosin/acrosin system

Moreno, RD; Barros, C

Abstract

Proacrosin is the zymogen of acrosin, a serine protease localized in the acrosomal matrix of mammalian sperm. Proacrosin/acrosin binds to solubilized zona pellucida glycoproteins (ZPGs) and various polysulfates in a non-enzymatic mechanism. In addition, both polysulfates and ZPGs induce proacrosin activation once they bind to the polysulfate-binding domain (PSBD) of the enzyme. We show here that the peptide (43)IFMYHNNRRYHTCCGILL(60) inhibited the proacrosin activation induced by either fucoidan or ZPGs. In addition, the peptide was recognized by the monoclonal antibody C5F10, which is directed against the PSBD region. Our data suggest that the PSBD is composed of many subsites that may or may not interact with each other.

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Título según WOS: A basic 18-amino acid peptide contains the polysulfate-binding domain responsible for activation of the boar proacrosin/acrosin system
Título según SCOPUS: A basic 18-amino acid peptide contains the polysulfate-binding domain responsible for activation of the boar proacrosin/acrosin system
Título de la Revista: BIOLOGY OF REPRODUCTION
Volumen: 62
Número: 6
Editorial: OXFORD UNIV PRESS INC
Fecha de publicación: 2000
Página de inicio: 1536
Página final: 1542
Idioma: English
URL: http://www.biolreprod.org/cgi/doi/10.1095/biolreprod62.6.1536
DOI:

10.1095/biolreprod62.6.1536

Notas: ISI, SCOPUS