Impaired biliary cholesterol secretion and decreased gallstone formation in apolipoprotein E-deficient mice fed a high-cholesterol diet

Amigo, L; Quiñones V.; Mardones P.; Zanlungo S.; Miquel, JF; Nervi, F; Rigotti, A.

Abstract

Background & Aims: Because apolipoprotein E (apoE) is a key cholesterol transport molecule involved in the hepatic uptake of chylomicron cholesterol, it may play a critical role in controlling bile cholesterol elimination and cholesterol gallstone formation induced by dietary cholesterol, To test this hypothesis, we studied biliary lipid secretion and gallstone formation in apoE-deficient mice fed cholesterol-rich diets. Methods: Bile lipid outputs and gallstone sequence events were analyzed in apoE-deficient mice fed a high-cholesterol diet or a lithogenic diet compared with control animals. Results: A high-cholesterol diet increased biliary cholesterol secretion and gallbladder bile cholesterol concentration in wild-type mice; the increase in bile cholesterol secretion was significantly attenuated in apoE-deficient mice. ApoE knockout mice fed a high-cholesterol lithogenic diet had a markedly lower frequency of gallbladder bile cholesterol crystal and gallstone formation than wild-type mice, which was most likely a result of the decreased cholesterol saturation index found in gallbladder bile of apoE-deficient mice. Conclusions: These results show that apoE expression is an important factor for regulating both biliary secretion of diet-derived cholesterol as well as diet-induced cholesterol gallstone formation in mice.

Más información

Título según WOS: Impaired biliary cholesterol secretion and decreased gallstone formation in apolipoprotein E-deficient mice fed a high-cholesterol diet
Título según SCOPUS: Impaired biliary cholesterol secretion and decreased gallstone formation in apolipoprotein E-deficient mice fed a high-cholesterol diet
Título de la Revista: GASTROENTEROLOGY
Volumen: 118
Número: 4
Editorial: W B SAUNDERS CO-ELSEVIER INC
Fecha de publicación: 2000
Página de inicio: 772
Página final: 779
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0016508500701478
DOI:

10.1016/S0016-5085(00)70147-8

Notas: ISI, SCOPUS