Environmental acclimatization of the carp modulates the transcription of beta-actin

Sarmiento J.; Leal, S.; Quezada C.; Kausel G.; Figueroa J.; Vera, MI; Krauskopf, M

Abstract

A cascade of mechanisms involving changes in gene expression are substantial to shape the adaptive responses that a eurythermal fish requires upon environmental changes in its habitat. We have previously shown that the cyclical reprogramming of rRNA transcription is a remarkable feature in carp under seasonal acclimatization. Using in situ hybridization and competitive RT-PCR we found significant differences in p-actin transcripts, generally accepted to be coded by a typical housekeeping gene, in tissues from summer- and winter-acclimatized carp. The physiological differential p-actin transcription herein reported places us on the alert for the reference genes estimated to be constitutive to quantitatively assess gene transcripts. J. Cell. Biochem. 80:223-228, 2000. (C) 2000 Wiley-Liss, Inc.

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Título según WOS: Environmental acclimatization of the carp modulates the transcription of beta-actin
Título de la Revista: JOURNAL OF CELLULAR BIOCHEMISTRY
Volumen: 80
Número: 2
Editorial: WILEY-BLACKWELL
Fecha de publicación: 2000
Página de inicio: 223
Página final: 228
Idioma: English
Notas: ISI