Cloning of a WD-repeat-containing gene from alfalfa (Medicago sativa): a role in hormone-mediated cell division?

McKhann, HI; Frugier, F; Petrovics, G; delaPena, TC; Jurkevitch, E; Brown, S; Kondorosi, E; Kondorosi, A; Crespi, M

Abstract

Rhizobium meliloti can interact symbiotically with Medicago plants thereby inducing the formation of root nodules. Screening of a young nodule cDNA library led to the isolation of a cDNA from Medica,oo sativa, Msgbl, that comprises a new member of the RACK1 (Receptor of Activated C Kinase) subfamily of WD-repeat proteins. This subfamily shows homology to the beta-subunit of heterotrimeric G proteins. Besides RACK1, this subfamily contains several plant genes including the well characterized auxin-inducible ArcA of tobacco. The Msgbl gene is strongly expressed in young embryos and in leaves, and is induced upon cytokinin treatment of roots. Whereas northern analysis failed to reveal differences in expression between total RNA from roots and nodules, in situ hybridization demonstrated that the transcript was most abundant in dividing cells of nodule primordia and in the nodule meristem. Msgbl may be related to the signal transduction acting in response to hormone-mediated cell division.

Más información

Título según WOS: ID WOS:A1997XR96200006 Not found in local WOS DB
Título de la Revista: PLANT MOLECULAR BIOLOGY
Volumen: 34
Número: 5
Editorial: Springer
Fecha de publicación: 1997
Página de inicio: 771
Página final: 780
DOI:

10.1023/A:1005899410389

Notas: ISI