A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine

Fonseca, Erica Lourenco; Ramos, Nilceia da Veiga; Nascimento Andrade, Bruno G.; Morais, Lena L. C. S.; Abanto Marin, Michel F.; Vicente, Ana Carolina P.

Abstract

Klebsiella pneumoniae, Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for bla(SHV), bia(LEN) and bla(OKP) and their flanking gene (deoR). Since this scheme focuses only on chromosomal genes, it will be feasible for Klebsiella identification in the clinical routine. (C) 2017 Elsevier Inc. All rights reserved.

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Título según WOS: ID WOS:000395617300004 Not found in local WOS DB
Título de la Revista: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volumen: 87
Número: 4
Editorial: Elsevier Science Inc.
Fecha de publicación: 2017
Página de inicio: 315
Página final: 317
DOI:

10.1016/j.diagmicrobio.2017.01.005

Notas: ISI