Effect of protein kinase C activation on the glycine evoked Cl- current in spinal cord neurons

Albarran, FA; Roa JP; Navarrete R.; Castillo R.; Nualart, F.; Aguayo, LG

Abstract

We investigated whether the effect of phorbol-12-myristate-13-acetate (PMA) was altered by a kinase inhibitor and by down-regulation of protein kinase C (PKC) in order to determine if glycine receptors in mouse spinal neurons, unlike those in hippocampal and trigeminal neurons, can be inhibited by PKC. To examine the above, electrophysiological and immunofluorescence studies were carried out in mouse spinal neurons kept in culture for up to 3 weeks. The inhibition of the glycine activated current by PMA (1 ?M) increased from 12±3% during week 1 to 27±6% during week 3. The effect of PMA was completely blocked by the PKC selective inhibitor RO 31-8220 (1 ?M). After culturing the cells with 1 ?M PMA for 24 h, the inhibitory effect of acute application of PMA disappeared altogether, suggesting that the effect of PMA was via PKC. Immunofluorescence studies showed that a short stimulation with PMA translocated the enzyme to the periphery whereas longer term stimulation (24 h) down regulated the PKC signal. These results indicate that activation of PKC by PMA inhibits the glycine receptor in cultured spinal neurons and that its sensitivity changes during neuronal development. © 2001 Elsevier Science B.V.

Más información

Título según WOS: Effect of protein kinase C activation on the glycine evoked Cl- current in spinal cord neurons
Título según SCOPUS: Effect of protein kinase C activation on the glycine evoked Cl- current in spinal cord neurons
Título de la Revista: BRAIN RESEARCH
Volumen: 902
Número: 1
Editorial: ELSEVIER SCIENCE BV
Fecha de publicación: 2001
Página de inicio: 1
Página final: 10
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0006899301022557
DOI:

10.1016/S0006-8993(01)02255-7

Notas: ISI, SCOPUS