Abstract

Reissner's fiber (RF) extends along the cerebral aqueduct, fourth ventricle, and the entire length of the central canal of the spinal cord. It grows continuously in the caudal direction by addition of newly released glycoproteins by the subcommissural organ (SCO) to its proximal end. Several hypotheses about RF function have been advanced. One of them postulates that RF binds biogenic amines present in the CSF and clears them away. In recent years, this hypothesis has been tested in our laboratory by using several experimental protocols. Firstly, the CSF concentration of monoamines was investigated in RF-deprived rats subjected to immunological neutralization of the SCO-RF complex. Secondly, the capacity of RF to bind monoamines in vivo was studied by injecting radiolabeled serotonin or noradrenaline into the rat CSF, and by perfusing them into the CSF, during one week, using an Alzet's osmotic pump. In vitro binding studies were performed using isolated bovine RF. All the findings obtained indicate that RF binds monoamines present in the ventricular CSF and then transports them along the central canal. In the absence of RF, the CSF concentration of monoamines increases sharply. © 2001 Wiley-Liss, Inc.