Cloning, expression and functional characterization of Schizosaccharomyces pombe TFIIB

Tamayo, E; Maldonado E.

Abstract

The transcription factor TFIIB has been identified and cloned from the yeast Schizosaccharomyces pombe. The cloned polypeptide is highly homologous to human TFIIB and to Saccharomyces cerevisiae TFIIB. S. pombe TFIIB is a 340-amino-acid-long protein and it possesses a repeated motif of 75 amino acids near the carboxy-terminal region. The purified recombinant protein is able to bind to the TBP-DNA promoter complex in gel retardation experiments. Recombinant S. pombe TFIIB is active in in vitro transcription assays, since it can complement the transcription activity of a S. pombe cell extract in which TFIIB was depleted by using antibodies. © 2002 Elsevier Science B.V. All rights reserved.

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Título según WOS: Cloning, expression and functional characterization of Schizosaccharomyces pombe TFIIB
Título según SCOPUS: Cloning, expression and functional characterization of Schizosaccharomyces pombe TFIIB
Título de la Revista: BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
Volumen: 1577
Número: 3
Editorial: ELSEVIER SCIENCE BV
Fecha de publicación: 2002
Página de inicio: 395
Página final: 400
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0167478102004426
DOI:

10.1016/S0167-4781(02)00442-6

Notas: ISI, SCOPUS