Secretion of endoxylanase A from Penicillium purpurogenum by Saccharomyces cerevisiae transformed with genomic fungal DNA

Chavez, R; Navarro, C.; Calderon, I; Peirano, A.; Bull, P; Eyzaguirre, J

Abstract

Saccharomyces cerevisiae was transformed with a genomic library from Penicillium purpurogenum, and an endoxylanase-producing yeast clone (named 44A) that grows on xylose or xylan as sole carbon source was isolated. This yeast synthesizes xynA mRNA and secretes endoxylanase A to culture media when grown on xylan or xylose, but not glucose. Analysis by pulse-field gel electrophoresis and sequencing indicates that xynA, including its eight introns, has been inserted into the yeast genome. It was shown by sequencing that clone 44A is able to correctly splice xynA introns. This is the first successful attempt to express a fungal endoxylanase gene in yeast with correct intron splicing. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

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Título según WOS: Secretion of endoxylanase A from Penicillium purpurogenum by Saccharomyces cerevisiae transformed with genomic fungal DNA
Título según SCOPUS: Secretion of endoxylanase A from Penicillium purpurogenum by Saccharomyces cerevisiae transformed with genomic fungal DNA
Título de la Revista: FEMS MICROBIOLOGY LETTERS
Volumen: 212
Número: 2
Editorial: OXFORD UNIV PRESS
Fecha de publicación: 2002
Página de inicio: 237
Página final: 241
Idioma: English
URL: http://doi.wiley.com/10.1111/j.1574-6968.2002.tb11272.x
DOI:

10.1111/j.1574-6968.2002.tb11272.x

Notas: ISI, SCOPUS