TGF-beta(1) and Smad4 overexpression induce a less invasive phenotype in highly invasive spindle carcinoma cells

Santibanez, JF; Quintanilla, M; Martinez, J.

Abstract

We have examined the effect of transforming growth factor ?1 (TGF-?1) and overexpression of the Smad4 gene on the phenotype of Car C, a ras mutated highly malignant spindle carcinoma cell line. TGF-?1-treated Car C cells overexpressing Smad4 spread with a flattened morphology with membrane ruffles abundant in vinculin and show a reduction in their invasive abilities. TGF-?1 treatment and overexpression of Smad4 also enhanced the production of PAI-1 measured by the activation of the p3TP-lux reporter gene containing a PAI-1-related promoter. This activation was abolished with a dominant-negative Smad4 construct. These results lead us to conclude that both TGF-?1 and Smad4 overexpression reduce the invasive potential of Car C cells, probably via the Smad pathway. © 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

Más información

Título según WOS: TGF-beta(1) and Smad4 overexpression induce a less invasive phenotype in highly invasive spindle carcinoma cells
Título según SCOPUS: TGF-?1 and Smad4 overexpression induce a less invasive phenotype in highly invasive spindle carcinoma cells
Título de la Revista: FEBS LETTERS
Volumen: 520
Número: 01-mar
Editorial: Elsevier
Fecha de publicación: 2002
Página de inicio: 171
Página final: 176
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0014579302027606
DOI:

10.1016/S0014-5793(02)02760-6

Notas: ISI, SCOPUS