Abstract

A simple differential equation model was developed to represent the growth and production of a secondary metabolite in solid-state cultivation (SSC) under conditions of limited nitrogen. The model was used to interpret data obtained from SSCs of the fungus Gibberella fujikuroi, under different temperatures (25 and 31 °C) and water activity conditions (0.985, 0.992, 0.999). The model was calibrated in two steps. An innovative procedure to achieve good initial guesses for key parameters, such as maintenance coefficients and death rates was first applied. These initial guesses were then used in a non-linear optimisation routine to get a minimum least squares fit for the model. The mathematical model was able to reproduce the measured variables: biomass, urea, starch, CO2, O2 and GA3 satisfactorily. Moreover, the model indicated that the fungus does not assimilate the nitrogen source, urea, directly. The model will be useful in developing optimal feeding policies and on-line biomass estimators. © 2002 Elsevier Science Ireland Ltd. All rights reserved.