Genomic analysis of the nitrate response using a nitrate reductase-null mutant of Arabidopsis

Wang, RC; Tischner, R; gutierrez ra; Hoffman, M; Xing, XJ; Chen, MS; Coruzzi, G; Crawford, NM

Abstract

A nitrate reductase (NR)-null mutant of Arabidopsis was constructed that had a deletion of the major NR gene NIA2 and an insertion in the NIA1 NR gene. This mutant had no detectable NR activity and could not use nitrate as the sole nitrogen source. Starch mobilization was not induced by nitrate in this mutant but was induced by ammonium, indicating that nitrate was not the signal for this process. Microarray analysis of gene expression revealed that 595 genes responded to nitrate (5 mm nitrate for 2 h) in both wild-type and mutant plants. This group of genes was overrepresented most significantly in the functional categories of energy, metabolism, and glycolysis and gluconeogenesis. Because the nitrate response of these genes was NR independent, nitrate and not a downstream metabolite served as the signal. The microarray analysis also revealed that shoots can be as responsive to nitrate as roots, yet there was substantial organ specificity to the nitrate response.

Más información

Título según WOS: ID WOS:000223962100010 Not found in local WOS DB
Título de la Revista: PLANT PHYSIOLOGY
Volumen: 136
Número: 1
Editorial: OXFORD UNIV PRESS INC
Fecha de publicación: 2004
Página de inicio: 2512
Página final: 2522
DOI:

10.1104/pp.044610

Notas: ISI