Fluorescent tagged analysis of neural gene function using mosaics in zebrafish and Xenopus laevis

Conway, G; Torrejon, M; Lin, S; Reinsch, S

Abstract

An important question in the neurosciences is the role of specific gene expression in the control of neural morphology and connectivity. To address this question, methods are needed for expression of exogenous genes in a subset of neurons. This limited and mosaic expression allows the assessment of gene expression in a cell autonomous fashion without environmental contributions from neighboring expressing cells. These methods must also label neurons so that detailed morphology and neural connections can be evaluated. The labeling method should label only a subset of neurons so that neuronal morphology can be viewed upon a non-stained background, in a Golgi staining fashion. Here, we report methods using plasmids called pTAGUM (tagged analysis of genes using mosaics) that accomplish these goals. These methods should prove useful for the analysis of neural gene function in two important model organisms, the zebrafish and Xenopus laevis. (c) 2005 Elsevier B.V. All rights reserved.

Más información

Título según WOS: ID WOS:000236099000018 Not found in local WOS DB
Título de la Revista: BRAIN RESEARCH
Volumen: 1070
Número: 1
Editorial: ELSEVIER SCIENCE BV
Fecha de publicación: 2006
Página de inicio: 150
Página final: 159
DOI:

10.1016/j.brainres.2005.11.079

Notas: ISI