Expression of the fructose transporter GLUT5 in human breast cancer

ZamoraLeon, SP; Golde, DW; Concha, II; Rivas, CI; DelgadoLopez, F; Baselga, J; Nualart, F; Vera, JC

Abstract

The primary metabolic characteristic of malignant cells is an increased uptake of glucose and its anaerobic metabolism. We studied the expression and function of the glucose transporters in human breast cancer cell lines and analyzed their expression in normal and neoplastic primary human breast tissue. Hexose uptake assays and immunoblotting experiments revealed that the breast carcinoma cell lines MCF-7 and MDA-468 express the glucose transporters GLUT1 and GLUT2, isoforms expressed in both normal and neoplastic breast tissue. We also found that the breast cancer cell lines transport fructose and express the fructose transporter GLUT5. Immunolocalization studies revealed that GLUT5 is highly expressed in vivo in human breast cancer but is absent in normal human breast tissue. These findings indicate that human breast cancer cells have a specialized capacity to transport fructose, a metabolic substrate believed to be used by few human tissues. Identification of a high-affinity fructose transporter on human breast cancer cells opens opportunities to develop novel strategies for early diagnosis and treatment of breast cancer.

Más información

Título según WOS: ID WOS:A1996TY96400021 Not found in local WOS DB
Título de la Revista: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volumen: 93
Número: 5
Editorial: NATL ACAD SCIENCES
Fecha de publicación: 1996
Página de inicio: 1847
Página final: 1852
DOI:

10.1073/pnas.93.5.1847

Notas: ISI