Immunopurification of Golgi vesicles by magnetic sorting

Mura, CV; Becker, ML; Orellana, A; Wolff, D

Abstract

We have designed a method that permits to isolate highly purified Golgi vesicles deprived of endoplasmic reticulum (ER), main contaminant of Golgi fractions. To this end, we prepared a rabbit polyclonal antibody against the cytosolic N-terminal oligopeptide of the enzyme heparan glucosaminyl N-deacetylase/N-sulphotransferase (HSST), a specific marker for Golgi apparatus. The Golgi localization of HSST was confirmed by indirect immunofluorescence microscopy. The antibody binding to Golgi vesicles was demonstrated by immunoelectronmicroscopy and allowed the immunopurification by magnetic sorting. Golgi vesicles subjected to purification by magnetic sorting showed the presence of HSST and p28, which is an integral membrane protein on the cis-Golgi also used as a specific Golgi marker. The purified material was devoid of calreticulin, a specific ER marker. This purification method will allow to improve studies requiring highly purified Golgi membranes such as identification of specific receptors and the electrophysiological characterization of Golgi membrane ion channels, which have been jeopardized up to now by ER membrane contamination. © 2002 Elsevier Science B.V. All rights reserved.

Más información

Título según WOS: Immunopurification of Golgi vesicles by magnetic sorting
Título según SCOPUS: Immunopurification of Golgi vesicles by magnetic sorting
Título de la Revista: JOURNAL OF IMMUNOLOGICAL METHODS
Volumen: 260
Número: 01-feb
Editorial: ELSEVIER SCIENCE BV
Fecha de publicación: 2002
Página de inicio: 263
Página final: 271
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0022175901005464
DOI:

10.1016/S0022-1759(01)00546-4

Notas: ISI, SCOPUS