Abstract

Assisted selection by molecular markers for fruit plants breeding is today a difficult challenge to achieve due to polygenic nature of the most agronomic traits. In many cases resulting in several QTLs for the same trait located in different chromosomes. Therefore it becomes increasingly necessary to use more efficient technologies, such as genotyping by sequencing (GBS), which allows generating saturated genetic maps and facilitate more accurate QTLs identification. The F1 Japanese plum population ‘98-99’ × ‘Angeleno’ of 153 seedlings was genotyped by GBS, using peach v1 as a reference genome, and resulting in a filtered data set of 42,909 SNPs. Genotyping errors, homozygotes and heterozygotes undercalling as well as SNPs with over 10% of missing data were eliminated. A total of 955 SNPs were mapped in ’98-99’ × ‘Angeleno’ family. Parental maps were constructed with 454 SNPs for ’98-99’ and 501 SNPs for ‘Angeleno’ covering a genomic region of 673.56 and 643.01cM respectively. Several phenological and agronomic traits were measured during 2016 season, finding major QTLs for ripening time, skin color and fruit weight in LG4, LG3 and LG7, respectively. These preliminary results represent a promising beginning for attempt molecular assisted selection as a tool in the new breeding programs of Japanese plum