Renibacterium salmoninarum iron-acquisition mechanisms and ASK cell line infection: Virulence and immune response

Bethke, Jorn; Arias-Munoz, Eloisa; Yanez, Alejandro; Avendano-Herrera, Ruben

Abstract

Renibacterium salmoninarum is the aetiological agent of bacterial kidney disease (BKD) in salmonid farms. This pathogen possesses at least three iron-acquisition mechanisms, but the link between these mechanisms and virulence is unclear. Therefore, this study used RT-qPCR to assess the effects of normal and iron-limited conditions on iron-uptake genes controlled by IdeR and related to iron acquisition in Chilean R. salmoninarum strain H-2 and the type strain DSM20767(T). Further evaluated was the in vitro immune-related response of the Atlantic Salmon Kidney (ASK) cell line, derived from the primary organ affected by BKD. R. salmoninarum grown under iron-limited conditions overexpressed genes involved in haemin uptake and siderophore transport, with overexpression significantly higher in H-2 than DSM20767(T). These overexpressed genes resulted in higher cytotoxicity and an increased immune response (i.e., TNF-alpha, IL-1 beta, TLR1 and INF-gamma) in the ASK cell line. This response was significantly higher against bacteria grown under iron-limited conditions, especially H-2. These observations indicate that iron-acquisition mechanisms are possibly highly related to the virulence and pathogenic capacity of R. salmoninarum. In conclusion, treatments that block iron-uptake mechanisms or siderophore synthesis are attractive therapeutic approaches for treating R. salmoninarum, which causes significant aquaculture losses.

Más información

Título según WOS: Renibacterium salmoninarum iron-acquisition mechanisms and ASK cell line infection: Virulence and immune response
Título según SCOPUS: Renibacterium salmoninarum iron-acquisition mechanisms and ASK cell line infection: Virulence and immune response
Título de la Revista: JOURNAL OF FISH DISEASES
Volumen: 42
Número: 9
Editorial: Wiley
Fecha de publicación: 2019
Página de inicio: 1283
Página final: 1291
Idioma: English
DOI:

10.1111/jfd.13051

Notas: ISI, SCOPUS