Novel Heterozygous Mutation in NFKB2 Is Associated With Early Onset CVID and a Functional Defect in NK Cells Complicated by Disseminated CMV Infection and Severe Nephrotic Syndrome

Aird, Alejandra; Lagos, Macarena; Vargas-Hernandez, Alexander; Posey, Jennifer E.; Coban-Akdemir, Zeynep; Jhangiani, Shalini; Mace, Emily M.; Reyes, Anaid; King, Alejandra; Cavagnaro, Felipe; Forbes, Lisa R.; Chinn, Ivan K.; Lupski, James R.; Orange, Jordan S.; Cecilia Poli, Maria

Abstract

Nuclear factor kappa-B subunit 2 (NF-kappa B2/p100/p52), encoded by NFKB2 (MIM: 164012) belongs to the NF-kappa B family of transcription factors that play a critical role in inflammation, immunity, cell proliferation, differentiation and survival. Heterozygous C-terminal mutations in NFKB2 have been associated with early-onset common variable immunodeficiency (CVID), central adrenal insufficiency and ectodermal dysplasia. Only two previously reported cases have documented decreased natural killer (NK) cell cytotoxicity, and little is known about the role of NF-kappa B2 in NK cell maturation and function. Here we report a 13-year-old female that presented at 6 years of age with a history of early onset recurrent sinopulmonary infections, progressive hair loss, and hypogamaglobulinemia consistent with a clinical diagnosis of CVID. At 9 years of age she had cytomegalovirus (CMV) pneumonia that responded to ganciclovir treatment. Functional NK cell testing demonstrated decreased NK cell cytotoxicity despite normal NK cell numbers, consistent with a greater susceptibility to systemic CMV infection. Research exome sequencing (ES) was performed and revealed a novel de novo heterozygous nonsensemutation in NFKB2 (c.2611C>T, p.Gln871*) that was not carried by either of her parents. The variant was Sanger sequenced and confirmed to be de novo in the patient. At age 12, she presented with a reactivation of the systemic CMV infection that was associated with severe and progressive nephrotic syndrome with histologic evidence of pedicellar effacement and negative immunofluorescence. To our knowledge, this is the third NF-kappa B2 deficient patient in which an abnormal NK cell function has been observed, suggesting a role for non-canonical NF-kappa B2 signaling in NK cell cytotoxicity. NK cell function should be assessed in patients with mutations in the non-canonical NF-kappa B pathway to explore the risk for systemic viral infections that may lead to severe complications and impact patient survival. Similarly NF-kappa B2 should be considered in patients with combined immunodeficiency who have aberrant NK cell function. Further studies are needed to characterize the role of NF-kappa B2 in NK cell cytotoxic function.

Más información

Título según WOS: Novel Heterozygous Mutation in NFKB2 Is Associated With Early Onset CVID and a Functional Defect in NK Cells Complicated by Disseminated CMV Infection and Severe Nephrotic Syndrome
Título según SCOPUS: Novel Heterozygous Mutation in NFKB2 Is Associated With Early Onset CVID and a Functional Defect in NK Cells Complicated by Disseminated CMV Infection and Severe Nephrotic Syndrome
Título de la Revista: Frontiers in Pediatrics
Volumen: 7
Editorial: FRONTIERS MEDIA SA
Fecha de publicación: 2019
Idioma: English
DOI:

10.3389/fped.2019.00303

Notas: ISI, SCOPUS