A DNA vaccine encoding Cu,Zn superoxide dismutase of Brucella abortus induces protective immunity in BALB/c mice

Onate, AA; Cespedes, S; Cabrera, A; Rivers, R; González A; Munoz, C; Folch H.; Andrews E.

Abstract

This study was conducted to evaluate the immunogenicity and protective efficacy of a DNA vaccine encoding Brucella abortus Cu,Zn superoxide dismutase (SOD). Intramuscular injection of plasmid DNA carrying the SOD gene (pcDNA-SOD) into BALB/c mice elicited both humoral and cellular immune responses. Animals injected with pcDNA-SOD developed SOD-specific antibodies which exhibited a dominance of immunoglobulin G2a (IgG2a) over IgG1. In addition, the DNA vaccine elicited a T-cell-proliferative response and also induced the production of gamma interferon, but not interleukin-10 (IL-10) or IL-4, upon restimulation with either recombinant SOD or crude Brucella protein, suggesting the induction of a typical T-helper-1-dominated immune response in mice. The pcDNA-SOD (but not the control vector) induced a strong, significant level of protection in BALB/c mice against challenge with B. abortus virulent strain 2308; the level of protection was similar to the one induced by B. abortus vaccine strain RB51. Altogether, these data suggest that pcDNA-SOD is a good candidate for use in future studies of vaccination against brucellosis.

Más información

Título según WOS: A DNA vaccine encoding Cu,Zn superoxide dismutase of Brucella abortus induces protective immunity in BALB/c mice
Título según SCOPUS: A DNA vaccine encoding Cu,Zn superoxide dismutase of Brucella abortus induces protective immunity in BALB/c mice
Título de la Revista: INFECTION AND IMMUNITY
Volumen: 71
Número: 9
Editorial: AMER SOC MICROBIOLOGY
Fecha de publicación: 2003
Página de inicio: 4857
Página final: 4861
Idioma: English
URL: http://iai.asm.org/cgi/doi/10.1128/IAI.71.9.4857-4861.2003
DOI:

10.1128/IAI.71.9.4857-4861.2003

Notas: ISI, SCOPUS