Large chloride channel from pre-eclamptic human placenta

Bernucci, L; Umana, F; Llanos P.; RIQUELME, G

Abstract

Chloride transport involving conductive pathways participates in numerous epithelial functions, such as membrane voltage maintenance, solute transport and cell volume regulation. Evidence points to involvement of transepithelial chloride transport in such functions in placental syncytiotrophoblast. A molecular candidate for physiologic conductive chloride transport in apical syncytiotrophoblast membrane is a Maxi-chloride channel with distinct biophysical properties: conductance over 200 Ps, multiple substates, voltage dependent open probability, and permeation to anionic amino acids. Pre-eclampsia, a high incidence pathology of pregnancy, exerts great impact on fetal morbi-mortality. This relies, among others, on intrauterine growth restriction (IUGR), thought to be mediated by diminished blood flow to the placenta, with growing knowledge regarding contribution of other factors. The Maxi-chloride channel's properties suggest it could be altered in this pathology. We have characterized the apical chloride channels from pre-eclamptie placentae, reconstituted in giant liposomes suitable for patch-clamp electrophysiological studies. In n=33 experiments from n=6 pre-eclamptie placentae we observed a chloridepermeable channel with similar biophysical properties to the channel from normal tissue (n=29 experiments from n=15 placentae). However, the main conductance state showed diminished magnitude (<150 Ps), and the open probability versus voltage relationship exhibited a flattened curve instead of the bell-shaped curve of normal placentae. These results arc the first evidence of a functionally altered ionic channel from placental syncytiotrophoblast in pre-eclampsia. Considering the abundance of chloride-conducting channel activity in human apical membrane and their relevance in epithelial function in general, these alterations could greatly disturb numerous placental functions that rely on syncytiotrophoblast integrity. © 2003 Published by Elsevier Ltd.

Más información

Título según WOS: Large chloride channel from pre-eclamptic human placenta
Título según SCOPUS: Large chloride channel from pre-eclamptic human placenta
Título de la Revista: PLACENTA
Volumen: 24
Número: 08-sep
Editorial: W. B. Saunders Co., Ltd.
Fecha de publicación: 2003
Página de inicio: 895
Página final: 903
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0143400403001449
DOI:

10.1016/S0143-4004(03)00144-9

Notas: ISI, SCOPUS