Abstract

The increase in CAV1 expression in hypoxia observed by indirect immunofluorescence (IFI) was quantified as the number of immunocompromised cells per field (data not shown), however we We proposed to quantify this increase by western blot using the HT29(US) and B16F10 exposed to hypoxia. As shown in Figure 2, B16F10(Mock) cells (A and B) and HT29(US)M1 (D and E) exposed to hypoxia have increased CAV1 expression compared to that observed in normoxia. It should be clarified that these hypoxia-induced levels do not reach the over expressed values since These are not only over-expressed but also IPTG-induced, which means that with this overexpression, very high levels of expression are reached. This high over-expression could explain, furthermore, why HT29(US)C14 and B16F10(Cav-1) cells exposed to hypoxia do not show increased levels of CAV1 than those exposed under normoxic conditions.