Abstract

We describe two new species of the genus Pristimantis from the western margin of the Eastern Cordillera of Colombia. The species have a sympatric distribution and are found in the humid tropical forests of the lower part of the Tama National Natural Park (PNN Tama) and its buffer zone. The new species were described from morphological comparisons and phylogenetic reconstruction from the sequencing of three mitochondrial and two nuclear genes. With these two new species, the number of Pristimantis species known in the department of Norte de Santander has increased to 10. In addition, we included new sequences for the species P. anolirex, P. nicefori, P. mondanifii, and P. yukpa. Our explorations in the last decade indicate that there arc probably between five and 10 species that have not been described in the northeastern part of Colombia, especially in areas where the armed conflict has predominated in the last five decades.