Abstract

The large-scale biomass production is an essential step in the biotechnological applications of microalgae. Coccomyxa onubensis is an acidophilic microalga isolated from the highly acidic waters of Rio Tinto (province of Huelva, Spain) and has been shown to accumulate a high concentration of lutein (9.7 mg g(-1)dw), a valuable antioxidant, when grown at laboratory-scale. A productivity of 0.14 g L-1 d(-1) was obtained by growing the microalga under outdoor conditions in an 800 L tubular photobioreactor. The results show a stable biomass production for at least one month and with a lutein content of 10 mg g(-1)dw, at pH values in the range 2.5-3.0 and temperature in the range 10-25 degrees C. Culture density, temperature, and CO2 availability in highly acidic medium are rate-limiting conditions for the microalgal growth. These aspects are discussed in this paper in order to improve the outdoor culture conditions for competitive applications of C. onubensis.