Caveolin-1 impairs PKA-DRP1-mediated remodelling of ER-mitochondria communication during the early phase of ER stress

Bravo-Sagua, R; Parra, V; Ortiz-Sandoval, C; Navarro-Marquez, M; Rodriguez, AE; Diaz-Valdivia, N; Sanhueza, C; Lopez-Crisosto, C; Tahbaz, N; Rothermel BA; Hill, JA; Cifuentes, M; Simmen, T; Quest, AFG; Lavandero, S

Abstract

Close contacts between endoplasmic reticulum and mitochondria enable reciprocal Ca2+ exchange, a key mechanism in the regulation of mitochondrial bioenergetics. During the early phase of endoplasmic reticulum stress, this inter-organellar communication increases as an adaptive mechanism to ensure cell survival. The signalling pathways governing this response, however, have not been characterized. Here we show that caveolin-1 localizes to the endoplasmic reticulum-mitochondria interface, where it impairs the remodelling of endoplasmic reticulum-mitochondria contacts, quenching Ca2+ transfer and rendering mitochondrial bioenergetics unresponsive to endoplasmic reticulum stress. Protein kinase A, in contrast, promotes endoplasmic reticulum and mitochondria remodelling and communication during endoplasmic reticulum stress to promote organelle dynamics and Ca2+ transfer as well as enhance mitochondrial bioenergetics during the adaptive response. Importantly, caveolin-1 expression reduces protein kinase A signalling, as evidenced by impaired phosphorylation and alterations in organelle distribution of the GTPase dynamin-related protein 1, thereby enhancing cell death in response to endoplasmic reticulum stress. In conclusion, caveolin-1 precludes stress-induced protein kinase A-dependent remodelling of endoplasmic reticulum-mitochondria communication.

Más información

Título según WOS: Caveolin-1 impairs PKA-DRP1-mediated remodelling of ER-mitochondria communication during the early phase of ER stress
Título de la Revista: CELL DEATH AND DIFFERENTIATION
Volumen: 26
Número: 7
Editorial: Nature Publishing Group
Fecha de publicación: 2019
Página de inicio: 1195
Página final: 1212
Idioma: English
DOI:

10.1038/s41418-018-0197-1

Notas: ISI