Olfactory transduction in ciliated receptor neurons of the Cabinza grunt, Isacia conceptionis (Teleostei : Haemulidae)

Schmachtenberg, O.; Bacigalupo, J

Abstract

The ciliated receptor neurons of fish olfactory organs are thought to transduce amino acids through a cAMP-dependent transduction pathway, but direct physiological evidence for this hypothesis remains scarce and is confined to catfish and trout. We investigated olfactory transduction in a marine fish, the Cabinza grunt Isacia conceptionis (Perciformes, Teleostei). The olfactory epithelium was characterized using light and electron microscopy, and isolated ciliated receptor neurons were recorded with the perforated patch-clamp technique. Cells were stimulated with puffer pipettes containing amino acid odourants, IBMX plus forskolin or 8bromo-cAMP. All three stimuli triggered transient inward currents at a holding potential of -70 mV and responses with outward-rectifying current-voltage relationships. The characteristics of the transduction currents induced by each stimulus were similar across cells and indistinguishable within the same cell, supporting the hypothesis of a cAMP pathway mediating transduction of amino acids in ciliated olfactory receptor neurons.

Más información

Título según WOS: Olfactory transduction in ciliated receptor neurons of the Cabinza grunt, Isacia conceptionis (Teleostei : Haemulidae)
Título según SCOPUS: Olfactory transduction in ciliated receptor neurons of the Cabinza grunt, Isacia conceptionis (teleostei: haemulidae)
Título de la Revista: EUROPEAN JOURNAL OF NEUROSCIENCE
Volumen: 20
Número: 12
Editorial: Wiley
Fecha de publicación: 2004
Página de inicio: 3378
Página final: 3386
Idioma: English
URL: http://doi.wiley.com/10.1111/j.1460-9568.2004.03825.x
DOI:

10.1111/j.1460-9568.2004.03825.x

Notas: ISI, SCOPUS