A Novel Saliva-Based miRNA Signature for Colorectal Cancer Diagnosis

Rapado-Gonzalez, O; Majem, B; Alvarez-Castro, A; Diaz-Pena, R; Abalo, A; Suarez-Cabrera, L; Gil-Moreno, A; Santamaria, A; Lopez-Lopez, R; Muinelo-Romay, L; Suarez-Cunqueiro, MM

Keywords: saliva, colorectal cancer, microrna, epigenomics, Liquid Biopsy, circulating biomarkers

Abstract

Salivary microRNAs (miRNAs) are of high interest as diagnostic biomarkers for non-oral cancer. However, little is known about their value for colorectal cancer (CRC) detection. Our study aims to characterize salivary miRNAs in order to identify non-invasive markers for CRC diagnosis. The screening of 754 miRNAs was performed in saliva samples from 14 CRC and 10 healthy controls. The differential expressed miRNAs were validated by RT-qPCR in 51 CRC, 19 adenomas and 37 healthy controls. Receiver operating characteristic (ROC) curves and logistic regression models were performed to analyze the clinical value of these miRNAs. Twenty-two salivary miRNAs were significantly deregulated in CRC patients vs. healthy individuals (p < 0.05) in the discovery phase. From those, five upregulated miRNAs (miR-186-5p, miR-29a-3p, miR-29c-3p, miR-766-3p, and miR-491-5p) were confirmed to be significantly higher in the CRC vs. healthy group (p < 0.05). This five-miRNA signature showed diagnostic value (72% sensitivity, 66.67% specificity, AUC = 0.754) to detect CRC, which was even higher in combination with carcinoembryonic antigen (CEA) levels. Overall, after the first global characterization of salivary miRNAs in CRC, a five-miRNA panel was identified as a promising tool to diagnose this malignancy, representing a novel approach to detect cancer-associated epigenetic alterations using a non-invasive strategy.

Más información

Título según WOS: A Novel Saliva-Based miRNA Signature for Colorectal Cancer Diagnosis
Título de la Revista: JOURNAL OF CLINICAL MEDICINE
Volumen: 8
Número: 12
Editorial: MDPI
Fecha de publicación: 2019
Idioma: English
DOI:

10.3390/jcm8122029

Notas: ISI