Development of a Two-Step Multiplex PCR Assay for Typing of Capsular Polysaccharide Synthesis Gene Clusters of Streptococcus suis

Okura, Masatoshi; lachance, ClauDe; Osaki, Makoto; Sekizaki, Tsutomu; Maruyama, Fumito; Nozawa, Takashi; Nakagawa, Ichiro; Hamada, Shigeyuki; Rossignol, Celine; Gottschalk, Marcelo; Takamatsu, Daisuke

Abstract

We developed a practical and easy two-step multiplex PCR assay to aid in serotyping of Streptococcus suis. The assay accurately typed almost all of the serotype reference strains and field isolates of various serotypes and also identified the genotypes of capsular polysaccharide synthesis gene clusters of some serologically nontypeable strains.

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Título según WOS: ID WOS:000337915700062 Not found in local WOS DB
Título de la Revista: JOURNAL OF CLINICAL MICROBIOLOGY
Volumen: 52
Número: 5
Editorial: AMER SOC MICROBIOLOGY
Fecha de publicación: 2014
Página de inicio: 1714
Página final: 1719
DOI:

10.1128/JCM.03411-13

Notas: ISI