Expression of the C-terminus of HIV-1 reverse transcriptase p66 and p51 subunits as a single polypeptide with RNase H activity

Zúñiga R.; Sengupta, S; Snyder, C.; Leon, O.; Roth, MJ

Abstract

The C-terminus of the HIV-1 reverse transcriptase heterodimer was reconstructed into a single polypeptide. The construct encodes the p51 thumb (T) and connection (C) subdomains joined through a linker region to the p66 connection (C) and RNase H (R) domain. The TCCR protein was purified from insoluble fractions of Escherichia coli lysates. The TCCR construct maintains Mn2+-dependent RNase H activity and specifically cleaves the substrate mimicking the tRNA removal required for second-strand transfer reactions.

Más información

Título según WOS: Expression of the C-terminus of HIV-1 reverse transcriptase p66 and p51 subunits as a single polypeptide with RNase H activity
Título según SCOPUS: Expression of the C-terminus of HIV-1 reverse transcriptase p66 and p51 subunits as a single polypeptide with RNase H activity
Título de la Revista: PROTEIN ENGINEERING DESIGN SELECTION
Volumen: 17
Número: 7
Editorial: OXFORD UNIV PRESS
Fecha de publicación: 2004
Página de inicio: 581
Página final: 587
Idioma: English
URL: http://peds.oxfordjournals.org/cgi/doi/10.1093/protein/gzh071
DOI:

10.1093/protein/gzh071

Notas: ISI, SCOPUS