Designing electrochemical interfaces based on nanohybrids of avidin functionalized-carbon nanotubes and ruthenium nanoparticles as peroxidase-like nanozyme with supramolecular recognition properties for site-specific anchoring of biotinylated residues

Gallay, Pablo; Eguilaz, Marcos; Rivas, Gustavo

Abstract

We are reporting an original supramolecular architecture based on a rationally designed new nanohybrid with enhanced peroxidase-like activity and site-specific biorecognition properties using avidin-functionalized multi-walled carbon nanotubes (MWCNTs-Av) and Ru nanoparticles (RuNPs). The nanohybrid-electrochemical interface was obtained by drop-coating of MWCNTs-Av dispersion at glassy carbon electrodes (GCE) followed by solvent evaporation and further electrodeposition of RuNPs (50 ppm RuCl2 for 15 s at -0.600 V). The simultaneous presence of MWCNTs and RuNPs produces a synergic effect on the non-enzymatic catatalytic reduction of H2O2 and allows the quantification of H2O2 in a wide linear range (from 5.0 x 10(-7) M to 1.75 x 10(-3) M) with a low limit of detection (65 nM). The avidin residues present in MWCNTs-Av/RuNPs hybrid nanomaterial allowed the anchoring by bioaffinity of biotinylated glucose oxidase (biot-GOx) as proof-of-concept of the analytical application of MWCNTs-Av platform for biosensors development. The resulting nanoarchitecture behaves as a bienzymatic-like glucose biosensor with a competitive analytical performance: linear range between 2.0 x 10(-5) M and 1.23 x 10(-3) M, sensitivity of (0.343 +/- 0.002) mu A mM(-1) or (2.60 +/- 0.02) mu A mM(-1) cm(-2), detection limit of 3.3 mu M, and reproducibility of 5.2% obtained with five different GCE/MWCNTs-Av/RuNPs/biot-GOx bioplatforms prepared the same day using the same MWCNTs-Av dispersion, and 9.1% obtained with nine biosensors prepared in different days with nine different MWCNTs-Av dispersions. The average concentrations of glucose in Gatorade, Red bull (R) and Pepsi (R) with the biosensor demonstrated excellent agreement with those reported in the commercial beverages.

Más información

Título según WOS: ID WOS:000501415300022 Not found in local WOS DB
Título de la Revista: BIOSENSORS BIOELECTRONICS
Volumen: 148
Editorial: ELSEVIER ADVANCED TECHNOLOGY
Fecha de publicación: 2020
DOI:

10.1016/j.bios.2019.111764

Notas: ISI